s pneumoniae r6 genomic dna Search Results


atcc strain baa 255d  (ATCC)
90
ATCC atcc strain baa 255d
Atcc Strain Baa 255d, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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emp2 genomic dna ccaatgccgtctctaatcacagctccaa r6 2030  (Thermo Fisher)
99
Thermo Fisher emp2 genomic dna ccaatgccgtctctaatcacagctccaa r6 2030
Mutant <t>emp2</t> Kernels Abort Early in Development.
Emp2 Genomic Dna Ccaatgccgtctctaatcacagctccaa R6 2030, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet28-r7-lbd kmr; pet28b(+) derivative containing dna fragment encoding r7-lbd  (GenScript corporation)
90
GenScript corporation pet28-r7-lbd kmr; pet28b(+) derivative containing dna fragment encoding r7-lbd
Mutant <t>emp2</t> Kernels Abort Early in Development.
Pet28 R7 Lbd Kmr; Pet28b(+) Derivative Containing Dna Fragment Encoding R7 Lbd, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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match candida albicans sc5314  (ATCC)
97
ATCC match candida albicans sc5314
Mutant <t>emp2</t> Kernels Abort Early in Development.
Match Candida Albicans Sc5314, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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granulibacter bethesdensis cgdnih1  (ATCC)
90
ATCC granulibacter bethesdensis cgdnih1
Mutant <t>emp2</t> Kernels Abort Early in Development.
Granulibacter Bethesdensis Cgdnih1, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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haemophilus ducreyi 35000hp  (ATCC)
92
ATCC haemophilus ducreyi 35000hp
Mutant <t>emp2</t> Kernels Abort Early in Development.
Haemophilus Ducreyi 35000hp, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptococcus suis sc84  (ATCC)
93
ATCC streptococcus suis sc84
Mutant <t>emp2</t> Kernels Abort Early in Development.
Streptococcus Suis Sc84, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tipifarnib  (Selleck Chemicals)
93
Selleck Chemicals tipifarnib
KEY RESOURCES TABLE
Tipifarnib, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neurospora crassa or74a  (ATCC)
94
ATCC neurospora crassa or74a
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Neurospora Crassa Or74a, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rhizobium etli cfn 42  (ATCC)
94
ATCC rhizobium etli cfn 42
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strain r6  (Eli Lilly)
86
Eli Lilly strain r6
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Strain R6, supplied by Eli Lilly, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s pneumoniae r6  (ATCC)
99
ATCC s pneumoniae r6
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S Pneumoniae R6, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mutant emp2 Kernels Abort Early in Development.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: Mutant emp2 Kernels Abort Early in Development.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques: Mutagenesis

DNA Gel Blot Analyses of Clones Linked to emp2.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: DNA Gel Blot Analyses of Clones Linked to emp2.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques: Western Blot, Clone Assay

Structural Map of the emp2 Gene.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: Structural Map of the emp2 Gene.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques:

RNA Gel Blot Analyses of emp2 Mutant Kernels.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: RNA Gel Blot Analyses of emp2 Mutant Kernels.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques: Western Blot, Mutagenesis

S1 Nuclease Protection Analyses of emp2 Transcripts.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: S1 Nuclease Protection Analyses of emp2 Transcripts.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques:

Oligonucleotide Primers Used in This Study

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: Oligonucleotide Primers Used in This Study

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques: Sequencing

EMP2 Is Homologous with Animal HSBP1.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: EMP2 Is Homologous with Animal HSBP1.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques:

EMP2 Protein Does Not Accumulate in Null emp2 Mutant Kernels.

Journal:

Article Title: empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

doi: 10.1105/tpc.006726

Figure Lengend Snippet: EMP2 Protein Does Not Accumulate in Null emp2 Mutant Kernels.

Article Snippet: The insertion positions have been deposited in GenBank. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name Primer Region Primer Sequence Probe 2 −11 to +25 of emp2 cDNA GGCAGGGTTTCGAAGGAACAAAGGCTGCTTGGC Probe 3 16 to 48 of emp2 cDNA GCGTCTCAGTGCCCATCTCAGCTTTGAGATCG Probe 4 392 to 421 of emp2 cDNA GGCTGCTTGGCCCAGGCAAAGCTCTGAGACGTTCGG Probe 5 135 to 171 of emp2 cDNA GGAGGAGGTACGGTGAGCGTGGGTGCGGCTCAGGTGG F1 297 to 316 of emp2 cDNA TCGAACCTTGTTTGCATCTG R1 524 to 545 of emp2 cDNA CAGATACGATCACGTACTGCTGA F2 1 to 21 of of emp2 cDNA AGAGCTTTG CCTGGGCCAA G R2 155 to 174 of emp2 cDNA TTCGGAGGAGGTACGGTGAG F3 3367 to 3388 of Zmdnaj TGCAAGGCTC TCGAGACAGT AC R3 3687 to 3666 of Zmdnaj CTTGCGAGGT CGATTCAGCT TC R4 281 to 308 of emp2 cDNA TGTTTGCATCTGTCCAAGGAGGTTCTGG R5 234 to 263 of emp2 cDNA TGTTGGGTTCTGTGATGATGGATCTGAATC F6 1868 to 1895 of emp2 genomic DNA CCAATGCCGTCTCTAATCACAGCTCCAA R6 2030 to 2057 of emp2 genomic DNA TCAGGTGGGTGGTTGTATACGAGTGGAA F7 5956 to 5982 of emp2 genomic DNA TGACTCTGCCTAACTTGGGTGTAACCC R7 6425 to 6452 of emp2 genomic DNA CCACTCCACAAGTCGAGGATATTCGTGT Open in a separate window Oligonucleotide Primers Used in This Study Maize Transcript Analyses For preparation of total RNA, 4 g of maize tissue was ground in liquid nitrogen with a mortar and pestle and thawed in Trizol lysis buffer (Gibco BRL).

Techniques: Mutagenesis

KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: A Pharmacogenomic Landscape in Human Liver Cancers

doi: 10.1016/j.ccell.2019.07.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Tipifarnib , Selleck Chemicals , Cat#S1453.

Techniques: Recombinant, Cell Viability Assay, Enzyme-linked Immunosorbent Assay, DNA Sequencing, RNA Sequencing, Drug discovery, Software, Control, Virus, Inhibition